Stress Responses of Genetically Modified Mucor Circinelloides Strains
نویسندگان
چکیده
The stress-related responses of Mucor circinelloides transformants altered in carotenoid production have been investigated. In zygomycetous fungi β-carotene is the predominant carotenoid, however, in transformants containing the crtW and crtZ astaxanthin biosynthesis genes from Agrobacterium aurantiacum new carotenoid compounds are present. Mucor strains with altered carotenoid content were treated with different concentrations of copper, cadmium, chromium and the oxidative stressinducing agents, menadione, tert-butyl hydroperoxide and hydrogen peroxide. Results suggest that the intermediers of the β-carotene-astaxanthin pathway more efficiently increase the stress tolerance of the fungal cells as the astaxanthin, the end-product of the biosynthesis.
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